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Servicebio Inc sds page loading buffer
Sds Page Loading Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds+page+loading+buffer/buffer+loading/pm42277000-573-6-9
Average 86 stars, based on 1 article reviews

sds page loading buffer - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Nanozyme-engineered liners for proactive prevention of wear particle-induced osteolysis.
Article Snippet: .. Protein extracts were mixed with 5× SDS-PAGE loading buffer (Servicebio #G2075) and heated at 100 °C for 5 min before storage. ..

Article Title: Repeated neonatal sevoflurane exposure causes adolescent anxiety/depression via microglial P2Y12R-Wnt/β-catenin pathway-mediated neurogenesis deficits.
Article Snippet: .. Subsequently, samples were denatured by boiling for 10 min at 100°C in 5× SDS-PAGE loading buffer (G2075, Servicebio). .. Equal amounts of protein (10 μg per lane) were separated by electrophoresis on 6-12% gradient SDS-polyacrylamide gels and then transferred onto PVDF membranes (IPVH00010, Millipore Sigma).

Article Title: Sleep deprivation disrupts lacrimal gland homeostasis via hypothalamic-pituitary-adrenal axis and gut dysbiosis in mice
Article Snippet: Protein concentrations were determined using a BCA assay kit (Cat. no. G2026-200T; Servicebio, China). .. Equal amounts of protein (5 μL per sample) were denatured with 5× SDS-PAGE loading buffer (Cat. no. G2075-100ML; Servicebio) and separated on 10% SDS-PAGE gels (Cat. no. G2043-50T; Servicebio). .. Proteins were transferred onto PVDF membranes (Cat. no. WGPVDF45; Servicebio) using a semi-dry transfer system (Cat. no. SVT-2; Servicebio) at 350 mA for 30 min. Membranes were blocked with 5% non-protein blocking buffer (Cat. no. G2052-500ML; Servicebio) for 30 min at room temperature and incubated overnight at 4 °C with primary antibodies against IL-17A (1:1000, Cat. no. GB11110; Servicebio, China) and ACTIN (1:5000, Cat. no. GB15003; Servicebio, China).

Article Title: Chlorpyrifos induced dysregulation of arginine biosynthesis pathway aggravates Alzheimer's disease progression in 5XFAD mice via microbiota-gut-brain axis crosstalk.
Article Snippet: The cortex and hippocampus tissues were fragmentated in pre-cooled RIPA buffer (Cat: G2002; Servicebio) for total protein extraction, followed by quantification of total protein concentration using a BCA protein assay kit (Cat: G2026; Servicebio). .. After denatured for 7 min at 95◦C in SDS-PAGE loading buffer (Cat: G2075; Servicebio), the same protein content was divided by 10% SDSPAGE and next shifted to 0.2 μm PVDF membranes. ..

Article Title: High-Fat Diet Induces Preeclampsia-Like Phenotypes Associated with Impaired Decidualization and NF-κB Activation.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: ALKBH5 demethylation modification of SE-lncRNA ZMIZ1-AS1 promotes FGFR1-mediated proliferation and invasive metastasis in osteosarcoma.
Article Snippet: Protein concentrations were determined using the Servicebio BCA Assay Kit (#G2026). .. Protein samples were denatured in SDS-PAGE loading buffer (Servicebio #G2075) at 100°C for 10 min, separated by 10% SDS-PAGE at 120 V, and then transferred onto PVDF membranes (Servicebio #G6044-0.45) at 400 mA. .. The membranes were blocked with NcmBlot blocking buffer (#P30500) for 15 min and subsequently incubated overnight at 4°C with the following primary antibodies: FOSL1 (1:1000; Abcam, #ab252421), ALKBH5 (1:5000; Proteintech, #67811-1-Ig), PTBP1 (1:4000; Proteintech, #12582-1-AP), and FGFR1 (1:2000; Proteintech, #60325-1-Ig).

Article Title: Influence of Consecutive Nab-Paclitaxel Chemotherapy Cycles on Gut Microbiota and Pharmacokinetic Behavior
Article Snippet: RIPA lysis buffer (Lot number G2002-100ML), protease inhibitor cocktail (Lot number G2006-250UL), PMSF (Lot number G2008-1ML), phosphatase inhibitor (Lot number G2007-1ML), and BCA protein assay kit (Lot number G2026-200 T) were obtained from Servicebio (China). .. SDS-PAGE loading buffer (Lot number G2075-100ML), precast gels (Lot number G2043-50T), prestained protein markers (Lot number G2087-250UL), PVDF membranes (Lot number WGPVDF45), blocking solution (Lot number G2052-500ML), and ECL chemiluminescence kits (Lot number G2014-50ML) were also purchased from Servicebio. .. Primary antibodies against CYP3A1 (Lot number pa3-034) and CYP2C11 (Lot number 231-330) were obtained from Thermo Fisher Scientific (USA) and Antibodies Online, respectively.

Article Title: Influence of Consecutive Nab-Paclitaxel Chemotherapy Cycles on Gut Microbiota and Pharmacokinetic Behavior.
Article Snippet: RIPA lysis buffer (Lot number G2002-100ML), protease inhibitor cocktail (Lot number G2006-250UL), PMSF (Lot number G2008-1ML), phosphatase inhibitor (Lot number G2007-1ML), and BCA protein assay kit (Lot number G2026-200 T) were obtained from Servicebio (China). .. SDS-PAGE loading buffer (Lot number G2075-100ML), precast gels (Lot number G2043-50T), prestained protein markers (Lot number G2087-250UL), PVDF membranes (Lot number WGPVDF45), blocking solution (Lot number G2052-500ML), and ECL chemiluminescence kits (Lot number G2014-50ML) were also purchased from Servicebio. .. Primary antibodies against CYP3A1 (Lot number pa3-034) and CYP2C11 (Lot number 231-330) were obtained from Thermo Fisher Scientific (USA) and Antibodies Online, respectively.

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$a , Domain architecture of RAP80 and ARISC constructs. FL, full-length; SIM, small ubiquitin-like modifier (SUMO)-interacting motif; UIM, ubiquitin-interacting motif; AIR, Abraxas1-interacting region; ZnF, zinc finger; MPN, Mpr1, Pad1 N-terminal; CC, coiled coil; UEV, ubiquitin E2 variant; vWFA, von Willebrand factor type A ( left ). Schematics of indicated complexes ( right ). b , SDS-PAGE analysis of ARISC, ARISC–RAP80, and ARISC–RAP80 AIR. c , K63-linked ubiquitin chains (1 µM) were incubated with ARISC or ARISC–RAP80 (5 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. d , K63-Ub2, -Ub4, and - Ub7 chains (1 µM) were incubated with ARISC, ARISC–RAP80, or ARISC–RAP80 AIR (5 nM) for the indicated time points. Cleavage activity was analysed as in c . Data are representative of three independent experiments. e , Schematics ( left ) and SDS-PAGE analysis ( right ) of indicated complexes. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. f , Alexa-Fluor 488 (AF488) labelled distally (AF488- Cys Ub4 K63R ) blocked K63-Ub4 chains (1.5 µM) were incubated with ARISC–RAP80, ARISC–RAP80 ΔUIMs, or ARISC–RAP80 ΔZnF (10 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and fluorescence scanning ( left ; see Methods ). The disappearance of the K63-Ub4 parent band was quantified using densitometry, and plotted as fraction of substrate consumed (%). Data points are mean ± SEM of two independent experiments ( right ). g , Cyclical and linear K63-Ub5 chains (2 µM) were incubated with ARISC or ARISC–RAP80 (10 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and Oriole staining. Data are representative of two independent experiments. Ub, ubiquitin; DUB, deubiquitylating enzyme. * indicates lower molecular weight ubiquitin species.$

Journal: bioRxiv

Article Title: Mechanism of K63-linked polyubiquitin recognition and cleavage by the BRCA1-A complex

doi: 10.64898/2026.06.05.730395

Figure Lengend Snippet: a , Domain architecture of RAP80 and ARISC constructs. FL, full-length; SIM, small ubiquitin-like modifier (SUMO)-interacting motif; UIM, ubiquitin-interacting motif; AIR, Abraxas1-interacting region; ZnF, zinc finger; MPN, Mpr1, Pad1 N-terminal; CC, coiled coil; UEV, ubiquitin E2 variant; vWFA, von Willebrand factor type A ( left ). Schematics of indicated complexes ( right ). b , SDS-PAGE analysis of ARISC, ARISC–RAP80, and ARISC–RAP80 AIR. c , K63-linked ubiquitin chains (1 µM) were incubated with ARISC or ARISC–RAP80 (5 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. d , K63-Ub2, -Ub4, and - Ub7 chains (1 µM) were incubated with ARISC, ARISC–RAP80, or ARISC–RAP80 AIR (5 nM) for the indicated time points. Cleavage activity was analysed as in c . Data are representative of three independent experiments. e , Schematics ( left ) and SDS-PAGE analysis ( right ) of indicated complexes. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. f , Alexa-Fluor 488 (AF488) labelled distally (AF488- Cys Ub4 K63R ) blocked K63-Ub4 chains (1.5 µM) were incubated with ARISC–RAP80, ARISC–RAP80 ΔUIMs, or ARISC–RAP80 ΔZnF (10 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and fluorescence scanning ( left ; see Methods ). The disappearance of the K63-Ub4 parent band was quantified using densitometry, and plotted as fraction of substrate consumed (%). Data points are mean ± SEM of two independent experiments ( right ). g , Cyclical and linear K63-Ub5 chains (2 µM) were incubated with ARISC or ARISC–RAP80 (10 nM) for the indicated time points. Cleavage activity was analysed by SDS-PAGE and Oriole staining. Data are representative of two independent experiments. Ub, ubiquitin; DUB, deubiquitylating enzyme. * indicates lower molecular weight ubiquitin species.

Article Snippet: Reactions were stopped with the addition of 3 μL 4x SDS-PAGE loading dye [240 mM Tris-HCl pH 6.8, 40% (v/v) glycerol, 8% (w/v) SDS, 0.04% (w/v) bromophenol blue, and 5% (v/v) β-Mercaptoethanol], and products were separated on 4-12% or 12% Nu-PAGE Bis-Tris gels (Invitrogen).

Techniques: Construct, Ubiquitin Proteomics, Variant Assay, SDS Page, Incubation, Activity Assay, Silver Staining, Fluorescence, Staining, Molecular Weight

a , K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or the indicated ARISC variants (5 nM) for 60 minutes. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. b, SDS-PAGE analysis of ARISC(E33A)–RAP80, ARISC(E33A) BRCC36(S98K) –RAP80, ARISC(E33A) Abraxas1(Δ42-55) –RAP80, and ARISC(E33A) BRCC45(ΔLoop) –RAP80. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. c, Spectral shift assays measuring binding of labelled ARISC(E33A)–RAP80 or the indicated mutant complexes (40 nM) to cyclical K63-Ub6 chains (20 µM-0 µM). Data points are mean ± SEM of two independent experiments carried out in technical duplicates. Dissociation constants (K d ) are indicated; CI, confidence interval. d, Representative images of WT or mutants BRCC36 IRIF in HT-29 cells 4 h post irradiation (10 Gy). Scale bar is 10 µm. e, Western blots showing BRCC36 protein levels in HT-29 cells reconstituted with WT or mutants BRCC36 as indicated (l eft ). Scatter plot showing quantification of the BRCC36 IRIF described in d . Data represent mean ± SEM derived from n ≥ 300 nuclei examined over two independent experiments; p values are indicated, unpaired two-tailed t test ( right ). f, K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or ARISC Δ42-55 (Abraxas1 Δ42-55) (5 nM) for up to 60 minutes. Cleavage activity was analysed as in a . Data are representative of two independent experiments. DUB, deubiquitylating enzyme; WT, wild type; Ub, ubiquitin.

Journal: bioRxiv

Article Title: Mechanism of K63-linked polyubiquitin recognition and cleavage by the BRCA1-A complex

doi: 10.64898/2026.06.05.730395

Figure Lengend Snippet: a , K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or the indicated ARISC variants (5 nM) for 60 minutes. Cleavage activity was analysed by SDS-PAGE and silver staining. Data are representative of two independent experiments. b, SDS-PAGE analysis of ARISC(E33A)–RAP80, ARISC(E33A) BRCC36(S98K) –RAP80, ARISC(E33A) Abraxas1(Δ42-55) –RAP80, and ARISC(E33A) BRCC45(ΔLoop) –RAP80. dStrepII, double StrepII tag. * indicates Abraxas1 degradation product. c, Spectral shift assays measuring binding of labelled ARISC(E33A)–RAP80 or the indicated mutant complexes (40 nM) to cyclical K63-Ub6 chains (20 µM-0 µM). Data points are mean ± SEM of two independent experiments carried out in technical duplicates. Dissociation constants (K d ) are indicated; CI, confidence interval. d, Representative images of WT or mutants BRCC36 IRIF in HT-29 cells 4 h post irradiation (10 Gy). Scale bar is 10 µm. e, Western blots showing BRCC36 protein levels in HT-29 cells reconstituted with WT or mutants BRCC36 as indicated (l eft ). Scatter plot showing quantification of the BRCC36 IRIF described in d . Data represent mean ± SEM derived from n ≥ 300 nuclei examined over two independent experiments; p values are indicated, unpaired two-tailed t test ( right ). f, K63-Ub2, -Ub4, and -Ub7 chains (1 µM) were incubated with ARISC WT or ARISC Δ42-55 (Abraxas1 Δ42-55) (5 nM) for up to 60 minutes. Cleavage activity was analysed as in a . Data are representative of two independent experiments. DUB, deubiquitylating enzyme; WT, wild type; Ub, ubiquitin.

Techniques: Incubation, Activity Assay, SDS Page, Silver Staining, Binding Assay, Mutagenesis, Irradiation, Western Blot, Derivative Assay, Two Tailed Test, Ubiquitin Proteomics

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